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Fig. 7 | Microbial Cell Factories

Fig. 7

From: The first report on the sortase-mediated display of bioactive protein A from Staphylococcus aureus (SpA) on the surface of the vegetative form of Bacillus subtilis

Fig. 7

Schematic representation of SpA display constructs. Plasmids were constructed to express the SpA with or without the C-terminal cell wall motif (CWM) of YhcR, and with or without the sortase YhcS. In the genome of B. subtilis, the stop codon of yhcR (TGA) and the start codon of yhcS (GTG) overlap. This feature was retained in our constructs to ensure that both genes are transcribed in a similar manner from the same promoter. a pSpA-YhcS is the main construct for SpA display on the B. subtilis cell wall surface, b the control construct named pNC1 lacks the sortase, c the control construct named pNC2 lacks both the CWM and sortase. SD Shine Dalgarno sequence; spa S. aureus protein A; CWM cell wall motif; YhcS: sortase of B. subtilis

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Microbial Cell Factories

ISSN: 1475-2859

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