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Fig. 5 | Microbial Cell Factories

Fig. 5

From: The first report on the sortase-mediated display of bioactive protein A from Staphylococcus aureus (SpA) on the surface of the vegetative form of Bacillus subtilis

Fig. 5

Purification of rabbit serum IgG by recombinant B. subtilis strains. IgG heavy chain is about 50 KDa and IgG light chain is about 23 KDa. Lane 1. Protein marker; Lane 2. Supernatant from serum incubation with the commercial protein A-agarose as a positive control; Lane 3. Supernatant from incubation of serum with the recombinant Bs-SpA strain; Lane 4. Supernatant from serum incubation with the recombinant Bs-pHY strain as a negative control

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Microbial Cell Factories

ISSN: 1475-2859

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