Fig. 3From: Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implicationsSummary of factors impacting the expression of difficult-to-express enzymes. A Parameters frequently explored and fine-tuned in experimental design, usually in an ad hoc manner; expression hosts and vector design. B Underlying factors that contribute to the formation of inclusion bodies and the bioinformatics and modelling tools used to evaluate the impact of these factors. The connecting lines demonstrate the interconnectivity of these parametersBack to article page