Fig. 5
Stability of whole-cell catalyst. A Whole-cell of BL21(DE3)/pET28a- INP-Faa. B Cell free part of BL21(DE3)/pET28a -Faa. The assays were carried out in Tris–HCL buffer (pH 7.8, 50 mM) containing 200 mM KF, 1 mM SAM and 8 OD600 induced cells of INP-Faa or cell-free extracts of Faa at equal quantity for 2 h at 50 ℃. Relative values were based on on catalytic ability at the initial time. Each value and error bar represents the mean of three independent experiments and its standard deviation