Fig. 3

The relative activity of different fractions to the cell fragmentation. A BL21(DE3)/pET28a- INP-Faa. B: BL21(DE3)/pET28a -Faa. The assays were carried out in Tris–HCL buffer (pH 7.8, 50 mM) containing 20 mM KF, 1 mM SAM and induced cells or cell fragmentation at equal quantity for 1 h at 37 ℃. Relative values were based on catalytic ability of the cell to the disrupted cell suspension. Results are presented as the mean values and standard deviations of data from three independent biological replicates