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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Fig. 3

The expression of the optimized sequences designed with codon usage and codon-pair context in Pichia pastoris. A, C The expression analysis of MT1-MMP E2C6 and ADAM17 A9B8 by Western blot using anti-his tag primary antibody. B, D The quantitative analysis to A, C. #1, #2 and #3 represent three different recombinant strains. M protein marker, CUB codon usage bias, CPB codon pair bias

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Microbial Cell Factories

ISSN: 1475-2859

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