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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Codon pair optimization (CPO): a software tool for synthetic gene design based on codon pair bias to improve the expression of recombinant proteins in Pichia pastoris

Fig. 2

The expression of variants of MT1-MMP E2C6 and ADAM17 A9B8 with the worst, the best, and the unbiased codon-pair context in Pichia pastoris. A, C Analysis of integrants using AOX1 general primers by PCR. Lane GS115 is a control using the host GS115 genome as the template, which show AOX1 gene, and lane pPIC9K-E2C6 or pPIC9K-A9B8 is another control using recombinant plasmid pPIC9K-E2C6 or pPIC9K-A9B8 as the template, which shows synthetic gene of MT1-MMP E2C6 and ADAM17 A9B8. B, D The expression of variants in Pichia pastoris. MT1-MMP E2C6 and ADAM17 A9B8 in the supernatant are detected by Western blot using anti-his tag primary antibody. #1, #2 and #3 represent three different recombinant strains. WCC the worst codon-pair context, BCC the best codon-pair context, UCC the unbiased codon-pair context

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Microbial Cell Factories

ISSN: 1475-2859

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