Table 1 Strains and plasmids used in this work
From: Improving CoQ10 productivity by strengthening glucose transmembrane of Rhodobacter sphaeroides
Strains and plasmids | Description | Reference or source |
|---|---|---|
Strains | ||
Wild-type | Rhodobacter sphaeroides ATCC 17025 | Lab preservation |
Δglk | glk markerless deletion mutant | This work |
ΔfruAΔfruB | fruAB markerless deletion mutant | This work |
ΔfruAΔfruB/bp | ΔfruAΔfruB harboring pBBR1MCS-2 | This work |
ΔfruAΔfruB/galPOP | ΔfruAΔfruB harboring pBBR1MCS-2:: galP | This work |
ΔfruAΔfruB/tac::galPOP | ΔfruAΔfruB harboring pBBR1MCS-2:: tac::galP | This work |
ΔfruAΔfruB/tac::glkOP | ΔfruAΔfruB harboring pBBR1MCS-2:: tac::glk | This work |
E. coli S17-1 | recA, harboring the genes tra, proA, thi-1(pRP4-2-Tc::Mu-Km::Tn7) | Lab preservation |
Plasmids | ||
pK18mobsacB | suicide vector, sacB (sucrose sensitivity), Kmr | Lab preservation |
pBBR1MCS-2 | ori pBBR1, lacZa, Kmr, used for geng over-expression | Lab preservation |
pBBR1MCS-2::tac | pBBR1MCS-2 harboring the tac promoter | Lab preservation |
pK18mobsacB::glk-L-R | For glk deletion | This work |
pK18mobsacB::fruA-L-R | For fruA deletion | This work |
pK18mobsacB::fruB-L-R | For fruB deletion | This work |
pBBR1MCS-2::galP | For galP expression | This work |
pBBR1MCS-2::tac::galP | For galP expression with strong promoter tac | This work |
pBBR1MCS-2::tac::glk | For glk expression with strong promoter tac | This work |