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Fig. 4 | Microbial Cell Factories

Fig. 4

From: A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae

Fig. 4

The effect of GAL1 and GAL80 double deletion on synthetic promoters. a Schematic diagram of galactose metabolism. GAL1: Galactokinase; GAL7: Galactose-1-phosphate uridyl transferase; GAL10: UDP-glucose-4-epimerase; GAL5: Phosphoglucomutase. UDP: Uridine diphosphate; EMP: Embden-Meyerhof-Parnas. In this study, GAL1 was deleted to block galactose metabolism. b Double deletion of GAL1 and GAL80 enhanced GAL promoter activity under glucose growth condition. c Double deletion of GAL1 and GAL80 improved synthetic promoter activity under raffinose or fructose conditions. d Galactose induction profile of promoters in GAL1/GAL80 double deletion strain. e Characterization of the synthetic promoter system under different carbon sources. Data are mean ± SD from three biological replicates and the shadow patterns of the curve represents errors as standard deviation

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Microbial Cell Factories

ISSN: 1475-2859

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