Fig. 4

Effect of glucose polymer boosting on cell growth and RH production. E. coli BQF8RH was cultivated in 3 mL EnPresso B medium with varying booster concentrations (0 ×, 0.25 ×, 0.5 ×, 1 ×, 2×) on 24-deepwell plate at 30 °C, 250 rpm for 30 h. RH expression was autoinduced by 50 µM IPTG or 2 g L⁻¹ lactose in the presence of 0.5 g L⁻¹ glucose. Samples normalized to OD₆₀₀ of 5 were taken at the end of cultivation and used for detection of HoxB levels by Western blotting (WB). A Cell growth, B WB for detection of HoxB yields, C specific HoxB yield and volumetric HoxB yield. Both specific and volumetric yield were calculated relative to the HoxB yield reached in 0 × boosted IPTG-autoinduced EnPresso culture (indicated by ♦)