|
Proteins
|
Cell growtha and protein titre
|
|---|
|
Wild-type (wt)
|
ΔdsbB
|
|---|
|
0 Cysc
|
100 Cys
|
0 Cys
|
100 Cys
|
|---|
|
scFv13R4
|
4.88 ± 0.08
|
4.91 ± 0.20
|
3.08 ± 0.10
|
3.50 ± 0.05
|
|
5.87 ± 0.21 mg/L
|
6.89 ± 0.37 mg/L
|
1.28 ± 0.24 mg/L
|
2.97 ± 0.14 mg/L
|
|
scFv13R4CM
|
4.45 ± 0.09
|
4.35 ± 0.09
|
2.90 ± 0.00
|
3.65 ± 0.00
|
|
6.42 ± 0.70 mg/L
|
6.15 ± 0.41 mg/L
|
1.28 ± 0.24 mg/L
|
3.42 ± 0.81 mg/L
|
|
RNase A
|
3.55 ± 0.05
|
3.60 ± 0.14
|
3.12 ± 0.00
|
3.39 ± 0.05
|
|
5.64 ± 1.05 mg/L
|
5.40 ± 0.36 mg/L
|
0.85 ± 0.12 mg/L
|
4.07 ± 0.89 mg/L
|
|
NGRP
|
n.d
|
n.d
|
3.90 ± 0.00
|
3.83 ± 0.06
|
| | |
6.66 ± 0.77 mg/L
|
7.46 ± 0.33 mg/L
|
- aFinal cell density was measured as Abs600 recorded 4 h after induction
- bTotal protein titre was determined from the Western blot quantification data (glycosylated and non-glycosylated), normalised and converted from sample OD600 of periplasmic fraction and sample volume for Western blot. For the final cell density and protein titre, the average of three biological replicates is shown and the errors indicate standard deviation. cell culture was grown under standard cultivation condition in shake flask 10:50 ml
- cCulture medium was added with or without 100 μM of cystine at the same time with inducers addition. n.d. not determined. The change (increasing or decreasing) of cell growth and protein titre relative to control (production in wt or ΔdsbB strain without cystine supplementation) was analysed by t-test with Welch’s correction (Additional file 2: Table S5)
