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Table 1 Yields of VFAs products from the B. subtilis strains after 72 h of incubation

From: Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis

Strain

VFAs* (unit: mol/mol‧glycerol)

Glycerol Utilization (mol)

Acetic acid

Lactic acid

Acetoin

2,3-BD

W168

0.07 ± 0.014

0.07 ± 0.011

0.09 ± 0.009

0.16 ± 0.004

0.09 ± 0.002

W168∆2

0.09 ± 0.005

0.08 ± 0.011

0.06 ± 0.002

0.15 ± 0.013

0.08 ± 0.004

W168∆3

0.12 ± 0.001

0.00 ± 0.000

0.02 ± 0.002

0.22 ± 0.004

0.09 ± 0.002

W168∆4

0.14 ± 0.015

0.00 ± 0.000

0.03 ± 0.011

0.23 ± 0.019

0.09 ± 0.007

  1. *The concentrations of diacetyl, ethanol, pyruvic, and succinic acid were < 0.01 mol/mol‧glycerol