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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Establishment of a co-culture system using Escherichia coli and Pichia pastoris (Komagataella phaffii) for valuable alkaloid production

Fig. 1

Metabolites biosynthesized in plants (a) and cells used for de novo stylopine production (b). a Various specialized metabolites are produced from common intermediates like reticuline, strictosidine, p-coumaric acid, squalene, and others. These intermediates are derived from central metabolism. b Reticuline-producing E. coli cells (AN2014 strain) produce (S)-reticuline using simple carbon sources such as glucose or glycerol, via three engineered pathways, namely (1) an l-tyrosine-overproducing pathway, (2) a dopamine and 3,4-dihydroxyphenylacetaldehyde (3,4-DHPAA) production pathway from l-tyrosine, and (3) a reticuline-producing pathway from dopamine. Stylopine-producing P. pastoris (B52 strain) produce (S)-stylopine from (S)-reticuline via three steps catalyzed by berberine bridge enzyme (BBE), cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2)

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Microbial Cell Factories

ISSN: 1475-2859

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