Fig. 3
Amino acid sequence of TanHcw and its phylogenetic tree. a Amino acid sequence of TanHcw. Prediction of the signal peptide by SignalP 5.0 program (http://www.cbs.dtu.dk/services/SignalP/). Signal peptide (Sec/SPI) was marked by an oblong box, and the cleavage site was also labeled with an arrowhead. Two conserved motifs (GXSXG and DXXDXXXD) were marked with a brown box. b The phylogenetic tree of TanHcw and other tannases. Multiple sequence alignment was carried out using ClustalW (https://www.genome.jp/tools-bin/clustalw). The phylogenetic tree was constructed by using MEGA7.0 software (http://www.megasoftware.net/). The bar represents 0.5 amino acid substitutions per site