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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Non-destructive quantification of anaerobic gut fungi and methanogens in co-culture reveals increased fungal growth rate and changes in metabolic flux relative to mono-culture

Fig. 8

AGF mass-normalized fluxes reveal significant upregulation of acetate (via the hydrogenosome) and ethanol (via cytosolic PFL) fluxes, and significant downregulation of lactate and succinate flux in co-cultures. Formate and hydrogen are consumed by M. thaueri and therefore do not accumulate in co-cultures. While xylan is consumed more quickly in co-culture, the flux of xylan into C. churrovis is equal in mono- and co-cultures. Fluxes for succinate in mono- and co-culture and lactate in co-culture assume metabolite concentrations of 0 mM at 24 h, as observed values were below the detection limit. Bolded metabolites are detectable via our HPLC method. Metabolites in blue or red (also starred) showed significantly greater flux in co-culture or mono-culture, respectively. Arrow thickness correlates qualitatively with mono-culture flux values. Xu5P xylulose-5-phosphate, G3P glyceraldehyde-3-phosphate, PEP phosphoenolpyruvic acid, OXAC oxaloacetic acid, ADP adenosine diphosphate, ATP adenosine triphosphate, NAD+ nicotinamide adenine dinucleotide (oxidized), NADH nicotinamide adenine dinucleotide (reduced), AcCoA acetyl coenzyme A, PFL pyruvate formate lyase, PFO pyruvate:ferredoxin oxidoreductase. Created with BioRender

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Microbial Cell Factories

ISSN: 1475-2859

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