Table 2 Bacterial strains used in this study

E. coli S17-1 λ pir

Conjugative donor strain for maintenance and transfer of the suicide pgl donor plasmid, pSECpgl

[78]

E. coli χ7122

Genome-sequenced spontaneous Nal^R APEC O78:H9 ST23 strain isolated from the liver of a turkey with colibacillosis. Template for amplifying ~ 1 kb upstream (IntUP) and downstream (IntDN) homology arms for integration of the pgl locus marked with a kanamycin resistance cassette between 3,997,780 and 3,997,791 on the genome

[45]

E. coli χ7122 pgl

χ7122 pgl integrant carrying C. jejuni pgl locus marked with a kanamycin resistance cassette between position 3,997,780 and 3,997,791 on the genome

This study

E. coli W3110

O-antigen negative strain widely used as a safe laboratory E. coli K-12 strain, wbbL-, ƛ-, rph-1, IN(rrnD-rrnE)1

[79]

E. coli SDB1

W3110-derivative, ΔwaaL, ΔwecA

[57]

E. coli CLM24 cedA::pglB

W3110-derivative, ΔwaaL with genomic integration of IPTG-inducible C. jejuni PglB in cedA

Abouelhadid et al., (manuscript in preparation)

E. coli DH5α

Commercial strain used for cloning

New England Biolabs, #C2987H