Table 3 Primers used in this study
From: Influence of B. subtilis 3NA mutations in spo0A and abrB on surfactin production in B. subtilis 168
Name | Sequence 5′–3′ | Purpose |
|---|---|---|
s1009 | CTGCCGTTATTCGCTGGATT | Amplification of amyE locus |
s1010 | AGAGAACCGCTTAAGCCCGA | |
s1055 | GGCGCCAAATGAGCTTTAATGGA | Amplification of abrB*-cat from B. subtilis IIG168-13 and abrB::erm from BKE00370 |
s1056 | TGACCGCTGTCAGGGCTTTT | |
s1070 | ACTGCGATTTTTGGGGGTGT | Amplification of abh::erm from BKE14480 |
s1071 | TCTCATATGACCACCTGCCG | |
s1221 | CGATATGGACACAAAGAAACC | Amplification of spo0A::erm from BKE24220 |
s1222 | CAATGACTGAAACTTATACGCTTG |