Table 1 Plasmids and strains

Plasmids

pRADN1

E. coli-D. radiodurans shuttle vector, chloramphenicol resistance

Hirofumi Ohba et al., 2005

pET-26b

E. coli expression vector, kanamycin resistance

Novagen

pMBIS

E. coli mevalonate pathway vector

Martin et al., 2003

pET26b-ps_Dr

pET-26b-ps_Dr

This study

pRAD-kP

pRADN1-P_katA-ps_Dr

This study

pRAD-gP

pRADN1-P_groE-ps_Dr

This study

pRAD-tP

pRADN1-P_tufA-ps_Dr

This study

pRAD-P-I

pRADN1-P_katA-ps_Dr-P_katA-ispA

This study

pRAD-P-I-D

pRADN1-P_katA-ps_Dr -P_katA-ispA-P_katA-dxs

This study

pRAD-P-D

pRADN1-P_katA-ps_Dr -P_katA-dxs

This study

pRAD-I

pRADN1-P_katA-ispA

This study

pRAD-P:G-D

pRADN1-P_katA- ps_Dr:ispA-P_katA-dxs

This study

pRAD-G:P-D

pRADN1-P_katA-ispA:ps_Dr-P_katA-dxs

This study

pET-26b- dr0862::kan^R

pET-26b-dr0862::kan^R

This study

pET-26b- dr1395::kan^R

pET-26b-dr1395::kan^R

This study

Strains

E. coli BL21(DE3)

F ֿ ompT hsdSB (rB ֿ mB ֿ) gal dcm rne131 λ(DE3)

Invitrogen

E. coli NM522

supE, thi, Δ(lac-proAB), hsd5 (r ֿ, m ֿ)

Promega

D. radiodurans R1

wild type

ATCC 13,939

WT kP

D. radiodurans pRAD-kP

This study

WT gP

D. radiodurans pRAD-gP

This study

WT tP

D. radiodurans pRAD-tP

This study

WT P-I

D. radiodurans pRAD-P-I

This study

WT P-I-D

D. radiodurans pRAD-P-I-D

This study

∆dr1395 I

D. radiodurans ∆dr1395 pRAD-I

This studys

∆dr1395 P-I

D. radiodurans ∆dr1395 pRAD-P-I

This study

∆dr1395 P-I-D

D. radiodurans ∆dr1395 pRAD-P-I-D

This study

∆dr0862

D. radiodurans ∆dr0862

This study

∆dr0862 kP

D. radiodurans ∆dr0862 pRAD-P

This study

∆dr0862 P-I

D. radiodurans ∆dr0862 pRAD-P-I

This study

∆dr0862 P-D

D. radiodurans ∆dr0862 pRAD-P-D

This study

∆dr0862 P-I-D

D. radiodurans ∆dr0862 pRAD-P-I-D

This study

∆dr0862 P:G-D

D. radiodurans ∆dr0862 pRAD-P:G-D

This study

∆dr0862 G:P-D

D. radiodurans ∆dr0862 pRAD-G:P-D

This study

E. coli EcpsB

E. coli BL21 pMBIS pET26b-ps_Dr

This study