Fig. 8
From: Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
Application of split GFP assay in screening of cutinase-GFP11 secretion. C. glutamicum pPBEx2-NprE-Cutinase-GFP11 and pPBEx2-Pel-Cutinase-GFP11 growth curves by backscattered light (A) and dissolved oxygen (B). Confidence tubes deviated from 24 biological replicates per strain. Each replicate was induced with IPTG individually triggered by backscatter signal. The period in which all samples were induced is highlighted in gray. Cells were harvested 4 h after induction and cutinase-GFP11 in supernatant was detected by split GFP and cutinase activity assay (C). Error bars deviated from 24 biological and two technical replicates. Fluorescence signal of split GFP assay was measured after 16 h incubation at 20 \(^\circ\)C