Fig. 7
From: Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
Correlation of split GFP assay and cutinase-GFP11 activity. C. glutamicum pPBEx2-NprE-Cutinase-GFP11 supernatant containing cutinase-GFP11 was diluted with different factors and the target protein was detected by split GFP and cutinase activity assay. Error bars for both assays deviated from 8 technical replicates. Fluorescence signal of split GFP assay was measured after saturation of the fluorescence signal after about 10.5 h incubation