Table 1 Strains and plasmids used in this work. The materials generated in this work are stored at the Joint BioEnergy Institute (JBEI) and can be accessed at https://public-registry.jbei.org with the registry ID numbers provided
From: Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol
Strain name | Genotype or description | Source | JBEI Registry ID |
|---|---|---|---|
Cg | Corynebacterium glutamicum ATCC 13,032 | ATCC | |
Cg ΔphdA | Corynebacterium glutamicum ΔphdA | This study | JPUB_018349 |
Cg PAD | Corynebacterium glutamicum wild type with pJAM89 | This study | JPUB_018350 |
Cg ΔphdA PAD | Corynebacterium glutamicum ΔphdA with pJAM89 | This study | JPUB_018352 |
Bs | Bacillus subtilis RIK1285 (strain 168 derivative) | TakaraBio | |
Bs PAD | Bacillus subtilis RIK1285 with pJAM90 | This study | JPUB_018354 |
Ba | Bacillus amyloliquefaciens ATCC 23,350 | ATCC | |
Ec | Escherichia coli DH5α | Zymo Research | |
Ec PAD | Escherichia coli DH5α with pJAM88 | This study | JPUB_018346 |
Plasmid name | Description | Source | JBEI Registry ID |
|---|---|---|---|
pK18mobsacB | suicide vector | ATCC | |
pZ8-Ptac | IPTG-inducible strong promoter expression vector | Addgene [6] | |
pHT08 | IPTG-inducible strong promoter expression vector | MoBiTec | |
pJAM88 | pZ8-Ptac with PAD codon-optimized for E. coli | This study | JPUB_018347 |
pJAM89 | pZ8-Ptac with PAD codon-optimized for C. glutamicum | This study | JPUB_018351 |
pJAM90 | pHT-08 with PAD codon-optimized for B. subtilis | This study | JPUB_018355 |