Table 3 Effect of FAD or/and catalase on catalytic performance parameters—FDCA concentration (mM) and yield (%), enzyme half-life (t1/2), and total turnover number (TTN)—for the production of FDCA from HMF (6 mM) by the HMFO WT and variants
Enzyme | FAD | Catalase | FDCA (mM) | FDCA (%) | t1/2 (h) | TTN |
|---|---|---|---|---|---|---|
WT | − | − | 1.4 | 23 | 16 | 1650 |
+ | − | 1.4 | 23 | 17 | 1680 | |
– | + | 2.1 | 35 | 178 | 2520 | |
+ | + | 3.1 | 53 | 210 | 3810 | |
V367R | – | − | 3.5 | 58 | 19 | 4200 |
+ | − | − | 59 | 18 | 4240 | |
– | + | 6.0 | 100 | 128 | 7200 | |
+ | + | 6.0 | 100 | 315 | 7200 | |
W466F | − | − | − | 8 | 23 | 605 |
+ | − | − | 12 | 27 | 880 | |
− | − | 4.8 | 80 | 35 | 5730 | |
+ | + | 4.0 | 67 | 49 | 4820 | |
V367R/W466F | − | − | − | 24 | 24 | 1740 |
+ | − | − | 37 | 34 | 2650 | |
− | − | 4.3 | 74 | 64 | 5350 | |
+ | + | 3.2 | 53 | 76 | 3800 | |
8BxHMFO | − | − | − | 87 | 26 | 6240 |
+ | − | − | 96 | 38 | 6900 | |
− | − | 6.0 | 100 | 204 | 7200 | |
+ | + | 6.0 | 100 | 315 | 7200 |