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Table 2 Plasmids used in this study

From: Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras

Name

ori

Inducible promoter

Inducer

Selection marker

References

pJH114

pMB1

Ptrc

IPTG

bla

[37]

pLEMO-HbpD-ESAT6

p15a

Prha

Rhamnose

cat

This study

pLEMO-HbpD-ELL

p15a

Prha

Rhamnose

cat

This study

pLEMO-HbpD-SpT2

p15a

Prha

Rhamnose

cat

This study

pLEMO-HbpD-SpT2-LL

p15a

Prha

Rhamnose

cat

This study

pLEMO-HbpD-SpT2-Calm

p15a

Prha

Rhamnose

cat

This study

pLEMO-HbpD-GFPnb

p15a

Prha

Rhamnose

cat

This study

pLEMO-UpaG-SpT2-Calm

p15a

Prha

Rhamnose

cat

This study

pLEMO-UpaG-GFPnb

p15a

Prha

rhamnose

cat

This study

pRha-HbpD-ELL

pMB1

Prha

Rhamnose

kan

This study

pRha-HbpD-SpT2-Calm

pMB1

Prha

Rhamnose

kan

This study

pRha-HbpD-GFPnb

pMB1

Prha

Rhamnose

kan

This study

pTUM4

p15a

Pcat

Constitutive

cat

[44]

pTUM2

p15a

Pcat

Constitutive

cat

This study

pTUM6

p15a

Pcat

Constitutive

cat

This study

pET28-SpC2-mScarlet

pMB1

PT7

IPTG

kan

Lab collection

pET20b-GFP-His6

pMB1

PT7

IPTG

bla

J.W. de Gier, Stockholm, Sweden

pEH3-GFP

pMB1

PlacUV5

IPTG

cat

Lab collection