Fig. 3
From: Functional characterization of a highly specific l-arabinose transporter from Trichoderma reesei
Kinetics determination of Trire2_104072 with electrophysiology. a–c Representative experiments of the kinetics studies for l-arabinose (a), d-xylose (b) or proton concentration (c) at − 50 mV. Kinetics for proton concentration were determined in the presence of 5 mM l-arabinose. The values were normalized to the lowest current obtained for each sugar at − 50 mV. Insets show the predicted Km (a, b) or K0.5 (c) values (points) and their mean (bar) from three different oocytes. Line shows the Michaelis–Menten (a, b) or Hill (c) kinetics prediction. d Normalized current as a function of pH at -50 mV. Points and error bars present mean and standard deviation between currents obtained from three different oocytes, and line shows the Hill kinetics prediction. Inset shows the predicted K0.5 values as pH, with presentation as in a–c