Table 1 Strains and plasmids used in the study
Strains and plasmids | Relevant characteristics | Source |
|---|---|---|
Strains | ||
E. coli Trans1-T1 | F-φ80(lacZ)ΔM15ΔlacX74hsdR (rk -, mk +) ΔrecA1398endA1tonA | Transgene company |
E. coli BW25113 | rrnBT14ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADL78 | Laboratory storage |
E. coli BW25113 ∆tnaA | wild type ∆tnaA | Laboratory storage |
EcSc5HTP | BW25113 ∆tnaA harboring pBAD-Sa5HTP | This work |
EcXc5HTP | BW25113 ∆tnaA harboring pBAD-Xc5HTP | This work |
EcHe5HTP | BW25113 ∆tnaA harboring pBAD-He5HTP | This work |
EcTf5HTP | BW25113 ∆tnaA harboring pBAD-Tf5HTP | This work |
EcCt5HTP | BW25113 ∆tnaA harboring pBAD-Ct5HTP | This work |
EcSaCOMT | BW25113 ∆tnaA harboring pBAD-SaCOMT | This work |
EcOsCOMT | BW25113 ∆tnaA harboring pBAD-OsCOMT | This work |
EcMEL1 | BW25113 ∆tnaA harboring pBAD-MEL1 | This work |
EcMEL2 | BW25113 ∆tnaA harboring pBAD-MEL2 | This work |
EcMEL3 | BW25113 ∆tnaA harboring pBAD-Xc5HTP and pZS-MEL1 | This work |
EcMEL4 | BW25113∆tnaA harboring pBAD-Xc5HTP and pZS-MEL2 | This work |
EcHFSaCOMT | BW25113∆tnaA harboring pZS-MEL1 |  |
EcHFOsCOMT | BW25113∆tnaA harboring pZS-MEL2 |  |
EcMEL5 | BW25113∆tnaA harboring pBAD-5HTPCOMTsa and pZS-PsmHF | This work |
EcMEL6 | BW25113∆tnaA harboring pBAD-5HTPCOMTos and pZS-PsmHF | This work |
EcMEL7 | BW25113∆tnaA harboring pBAD-XcP4H-OsCOMT and pZS-SaPsmHF-phhBfolM | This work |
EcMEL7-1 | EcMEL7 + over expression of metk | This work |
EcMEL7-2 | EcMEL7 + over expression of mtn | This work |
EcMEL7-3 | EcMEL7 + over expression of luxS | This work |
EcMEL7-4 | EcMEL7 + over expression of mtn and luxS | This work |
EcMEL7-5 | EcMEL7∆speD | This work |
EcMEL7-6 | EcMEL7∆dcm | This work |
EcMEL8 | BW25113∆tnaA∆speD harboring pBAD-XcP4H-OsCOMT2 and pZS-SaPsmHF-phhBfolM | This work |
EcMELCXPM | BW25113∆tnaA∆speD harboring pBAD-OsCOMT2-XcP4H-PhhB-FolM and pZS-SaPsmHF | This work |
EcMELCX | BW25113∆tnaA∆speD harboring pBAD-OsCOMT2-XcP4H and pZS-SaPsmHF-PhhB-FolM | This work |
EcMELCS | BW25113∆tnaA∆speD harboring pBAD-OsCOMT2 and pZS-XcP4H -SaPsmHF-PhhB-FolM | This work |
EcMELCT7S | BW25113∆tnaA∆speD (DE3) harboring pBAD-PT7OsCOMT2 and pZS-XcP4H -SaPsmHF-PhhB-FolM | This work |
EcMELCtacS | BW25113∆tnaA∆speD harboring pBAD-PtacOsCOMT2 and pZS-XcP4H -SaPsmHF-PhhB-FolM | This work |
Plasmids | ||
pKD46 | AmpR, λ-Red recombinase expression plasmid, ara-inducible expression, temperature sensitive replication | Laboratory storage |
pKD13 | KanR, oriR plasmid containing an FRT-aph-FRT cassette | Laboratory storage |
pCP20 | AmpR, CmR, repA(Ts), pSC101 based vector expressing the yeast Flp recombinase | Laboratory storage |
pBAD/HisA | AmpR, pBR322 origin, araBAD promoter, araC gene | Laboratory storage |
pZS | CmR, p15A origin, pBAD based vector expressing recombinase | Laboratory storage |
pBAD-Sa5HTP | pBAD containing SaP4H, phhB, FolM genes | This work |
pBAD-Xc5HTP | pBAD containing XcP4H, phhB, FolM genes | This work |
pBAD-He5HTP | pBAD containing HeP4H, phhB, FolM genes | This work |
pBAD-Tf5HTP | pBAD containing TfP4H, phhB, FolM genes | This work |
pBAD-Ct5HTP | pBAD containing CtP4H, phhB, FolM genes | This work |
pBAD-SaCOMT | pBAD containing SaCOMT | This work |
pBAD-OsCOMT | pBAD containing OsCOMT | This work |
pBAD-MEL1 | pBAD containing SaP4H, phhB, FolM, SaPsmH, SaPsmF, SaCOMT | This work |
pBAD-MEL2 | pBAD containing SaP4H, phhB, FolM, SaPsmH, SaPsmF, OsCOMT | This work |
pZS-MEL1 | pZS containing SaPsmH, SaPsmF, SaCOMT | This work |
pZS-MEL2 | pZS containing SaPsmH, SaPsmF, OsCOMT | This work |
pBAD-5HTPCOMTsa | pBAD containing SaP4H, phhB, FolM, SaCOMT | This work |
pBAD-5HTPCOMTos | pBAD containing SaP4H, phhB, FolM, OsCOMT | This work |
pZS-PsmHF | pZS containing SaPsmH, SaPsmF | This work |
pBAD-XcP4H-OsCOMT | pBAD containing SaP4H, OsCOMT | This work |
pBAD-XcP4H-OsCOMT2 | pBAD containing SaP4H, OsCOMT (C303F, V321T) | This work |
pZS-SaPsmHF-phhBfolM | pZS containing SaPsmH, SaPsmF, phhB, FolM | This work |
pBADOsCOMT2-XcP4H-PhhB-FolM | pBAD containing OsCOMT2, XcP4H, PhhB, FolM | This work |
pBADOsCOMT2-XcP4H | pBAD containing OsCOMT2, XcP4H | This work |
pBAD-XcP4H-OsCOMT2 | pBAD containing XcP4H, OsCOMT2 | This work |
pBAD-OsCOMT2 | pBAD containing OsCOMT2 | This work |
pBAD-PT7OsCOMT2 | pBAD containing PT7-OsCOMT2 | This work |
pBAD-PtacOsCOMT2 | pBAD containing Ptac-OsCOMT2 | This work |