Fig. 3
Enhanced production of 2′-FL by putative α-1, 2-FT from B. cereus. A Production of 2′-FL by engineered S. cerevisiae with different α-1, 2-FTs. Strains expressing α-1, 2-FTs from different organisms, including FutC from H. pylori (FL04) and selected α-1, 2-FTs from Acetobacter sp. (FL05), B. cereus (FL06), B. eggerthii (FL07), B. unifomis (FL08), and N. californiae (FL09) were cultured in YP medium containing glucose 2% (w/v) and 0.12 g/L adenine. Galactose 3% (w/v) and lactose 0.2% (w/v) were added at 24 h and cultured for another 48 h. 2′-FL were quantified by HPLC. B LC-MS analysis of 2′-FL produced by FL06. C 1H-NMR spectrum of 2′-FL purified from FL06 fermentation broth