Fig. 2

Production of GDP-l-fucose (A) and 2′-FL (B) by the engineered strains. To produce GDP-l-fucose, strains FL01 and FL03 were cultured in YP medium containing 2% (w/v) glucose and 2% (w/v) galactose for 48 h. To produce 2′-FL, strain FL04 expressing FutC from H. pylori and the control strain FL03 were cultured in YP medium containing glucose 2% (w/v) and 0.12 g/L adenine. Galactose 3% (w/v) and lactose 0.2% (w/v) were added at 24 h and cultured for another 48 h. 2′-FL were quantified by HPLC