Fig. 4
Shake-flask cultivation profile of C. glutamicum harboring pZ8-paaH-ter-paaJ-paaF-tesB. a Cultivation parameters include OD600 (filled circles) and residual sugar (empty circles). N = 3; error bars = standard deviation. b Ion (m/z = 363) extracted chromatogram obtained by GC/MS of the cultivation broth (t = 55 h) of C. glutamicum harboring pZ8-paaH-ter-paaJ-paaF-tesB (black line) and an empty vector (grey line). The peak corresponding to 3-hydroxyadipate is indicated with a black arrow. c Cultivation profile of C. glutamicum harboring pZ8-Ptac showing OD600 (filled circles) and residual sugar (empty circles). N = 3; error bars = standard deviation. (d) Ion (m/z = 145.0506) extracted chromatogram obtained by LC/MS of adipic acid standard (purple), cultivation broth of C. glutamicum harboring pZ8-paaH-ter-paaJ-paaF-tesB (green), pZ8-paaH-tesB-paaJF (grey), and an empty vector (black)