Fig. 2

Biosynthesis of adipic acid pathway intermediates from glucose. Shake-flask cultivation profile of C. glutamicum harboring (a) pZ8-paaJ-tesB and (b) pZ8-paaH-tesB-paaJ. OD₆₀₀ (filled circles), residual sugar (empty circles). N = 3; error bars = standard deviation. c Representative GC/MS ion (m/z = 363) extracted chromatogram of MSTFA-derivatized cultivation broth from C. glutamicum transformed with pZ8-paaH-tesB-paaJ. The peak at retention time 13.3 min corresponds to MSTFA-derivatized 3-hydroxyadipic acid. (d) m/z fragmentation pattern of the peak corresponding to MSTFA-derivatized 3-hydroxyadipic acid from (c)