Table 2 Plasmids used in this study
Plasmid | Description | References |
|---|---|---|
pRS42H | 2µ origin | EUROSCARF |
p42H_gHIS3 | pRS42H carrying HIS3 disruption gRNA cassette | This study |
pRS426GPD | URA3, GPD promoter, CYC1 terminator, 2µ origin, and Amp | Mumberg et al. [45] |
p426_Bp_W/OSP | pRS426GPD harboring BpGH16A from B. plebeius, deletion signal peptide | This study |
p426_Bp_CL | pRS426GPD harboring BpGH16A and chicken lysozyme signal peptide | This study |
p426_Bp_αMF | pRS426GPD harboring BpGH16A and α-mating factor signal peptide | This study |
p426_Bp_STA1 | pRS426GPD harboring BpGH16A and STA1 signal peptide | This study |
p426_Bp_SED1 | pRS426GPD harboring BpGH16A and SED1 signal peptide | This study |
Cas9-NAT | p414-TEF1p-Cas9-CYC1t-NAT1 | Zhang et al. [34] |
pRS42K | 2µ origin, KanMX | Taxis and Knop [46] |
p42K_CS5 | pRS42K, gRNA cassette targeting the intergenic site on Chr XV | This study |
16Â A-D-CS5 | BpGH16A, SED1 signal peptide, donor DNA for CS5 site integration | This study |