Fig. 1

Schematic representation of NAOS production by engineered S. boulardii. BpGH16A expression was performed using two different systems. The first was a vector system with an auxotrophic marker, and the second was the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system. NAOSs, neoagarooligosaccharides; BpGH16A, endo-type β-agarase originating from human gut bacterium Bacteroides plebeius