Table 2 Absolute quantification of copies of residual plasmid PF1680 in a purified RNase PF enzyme preparation. Quantification was based on a standard curve generated by amplification of standard plasmids containing the ampicillin resistance gene
From: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
Sample | Dilution | Average calculated Plasmid copy number per µL |
|---|---|---|
Before Membrane adsorber | Undiluted | 1.17 × 107 |
1:10 | 8.45 × 106 | |
1:100 | 3.15 × 106 | |
After Membrane adsorber | Undiluted | 5 × 102 |
1:10 | 5.1 × 102 |