Table 1 Accumulation of RNase activity in a reduced volume through successive steps starting from extracellular medium up to elution from a cation exchange chromatography column
From: Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification
Downstream stage | Cell-free supernatant | Retentate ultrafiltration | Retentate diafiltration | Activity-containing elution fraction SP Seph FF |
|---|---|---|---|---|
Total protein concentration (µg mL − 1) | 254 | 1761 | 1332 | 226 |
Volume (mL) | 7000 | 647 | 660 | 332 |
Protein amount (mg) | 1778 | 1139 | 879 | 75 |