Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

Table 1 Therapeutic antibody fragments used in this study

Generic name	Type	Structure^a	Size	Isoelectric point	Disulfide bonds	Target	Indication
Nanobody (Nan, Camelid)	VhH	Single monomeric V_H	17 kDa	5.92	2	Lysozyme	Rheumatoid arthritis and Crohn´s disease [30]
Pexelizumab (Pex, Humanized)	scFv	V_H and V_L connected by a polypeptide linker^b	31 kDa	8.62	Uncertain	Complement C5	Coronary artery bypass and Angioplasty [31]
Ranibizumab (Ran, Humanized)	Fab	V_H, V_L, C_H1 and C_L	51 kDa	6.99	5	VEGF-A	Macular degeneration [32]

^aV_H, variable domain of heavy chain; V_L, variable domain of heavy chain; C_H1, the first constant domain of heavy chain; C_L, constant domain of light chain
^bThe sequence of polypeptide linker is GGGGSGGGGSGGGGS

ISSN: 1475-2859