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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Augmented peroxisomal ROS buffering capacity renders oxidative and thermal stress cross-tolerance in yeast

Fig. 5

The gene knockdown of CAT. a The schematic of gene knockdown was showed in the left side, the dCas9 with Mxi1 repressor was targeted to the N-terminus of CAT to repress the function of CAT. As showed in the right side, the efficiency of gene knockdown experiment was detected by RT-qPCR. b The catalase activity was measured and compared to assess the repression efficiency of dCas9 system. c Spot assay to test the impact caused by gene knockdown of CAT on cell growth of G14 at different temperature and with/without H2O2

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Microbial Cell Factories

ISSN: 1475-2859

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