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Table 4 Apparent kinetic parameters of the purified AcmB and KSTD1

From: Universal capability of 3-ketosteroid Δ1-dehydrogenases to catalyze Δ1-dehydrogenation of C17-substituted steroids

Substrate

AcmB

KSTD1

kcat/Km [M−1 s−1]

kcat/Km [M−1 s−1]

Androst-4-en-3,17-dione (1)

(4.00 ± 0.04)∙106

(5.17 ± 0.03)∙106

Cholest-4-en-3-one (2)

(9.25 ± 0.17)∙106

(4.71 ± 0.03)∙105

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Microbial Cell Factories

ISSN: 1475-2859

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