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Table 3 Apparent kinetic parameters of the purified AcmB

From: Universal capability of 3-ketosteroid Δ1-dehydrogenases to catalyze Δ1-dehydrogenation of C17-substituted steroids

Substrate

KmS [μM]

KmDCPIP [μM]

Vmax [μM min−1]

kcat [s−1]

kcat/KmS [M−1 s−1]

Androst-4-en-3,17-dione (1)

529.2 ± 32.5

223.5 ± 16.9

859.2 ± 45.5

46.1 ± 2.3

(0.87 ± 0.05)∙105

Cholest-4-en-3-one (2)

23.7 ± 1.16

37.3 ± 3.3

104.6 ± 2.7

5.8 ± 0.2

(2.45 ± 0.01)∙105

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Microbial Cell Factories

ISSN: 1475-2859

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