Table 1 Gibbs free energy for reactions
From: Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production
No. | Reactions and pathways | Enzyme | ΔrG (kJ/mol) | Keq |
|---|---|---|---|---|
1 | Glycerol + NAD+ = DHA + NADH + H+ | DhaD | 24.6 ± 3.6 | 4.9 × 10–5 |
2 | DHA + ATP = DHA-P + ADP | Dhak | − 13.7 ± 4.3 | 255 |
3 | DHA + PEP = DHA-P + pyruvate | Dhak123 | − 41.4 ± 4.4 | 1.8 × 107 |
4 | DHA-P + H2O = DHA + phosphate | HdpA | − 12.7 ± 4.3 | 166 |
1 + 2 | ATP + NAD+ + Glycerol = NADH + ADP + DHA-P | DhaD, Dhak | 10.9 | |
1 + 3 | PEP + NAD+ + Glycerol = NADH + pyruvate + DHA-P | DhaD, Dhak123 | − 16.8 | |
4–1 | NADH + DHA-P = NAD+ + Glycerol + phosphate | DhaD, HdpA | − 37.3 |