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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enzyme-catalyzed biodegradation of penicillin fermentation residues by β-lactamase OtLac from Ochrobactrum tritici

Fig. 3

Expression and purification of OtLac (SDS-PAGE of expression and purification of OtLac, protein samples were taken at different stages of expression and purification. Lane M, prestained protein marker. Lane 1, cell lysate before induction with IPTG. Lane 2, cell lysate after 24 h of expression. Lane 3, cell free supernatant after sonication. Lane 4, precipitation of cells. Lane 5, Proteins that do not bind to Ni2+ gel. Lane 6, 2 mM imidazole eluent. Lane 7 10 mM imidazole eluent. Lane 8, purified OtLac) with 50 mM imidazole eluent

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Microbial Cell Factories

ISSN: 1475-2859

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