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Table 3 Effects of rhIL-2, rhIL-15 and IL-15/SuIL-15Rα-IgG4 Fc complexes on CD8+ T cell phenotypic markers

From: Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives

Marker

Ctrl

rhIL-2

rhIL-15

dFc

mFc

IFN-γ

1.6 ± 0.3

1.7 ± 0.4

6.5 ± 2.3

8.6 ± 4.1

8.4 ± 3.1

Granzyme B

20.7 ± 7.5

22.9 ± 8.3

24.5 ± 7.4

25.3 ± 7.7

25.4 ± 8.0

Perforin

31.8 ± 7.3

37.5 ± 7.7

40.7 ± 10.4

40.6 ± 11.9

40.1 ± 11.8

Ki67

0.9 ± 0.4

1.5 ± 0.7

2.6 ± 0.9

3.2 ± 2.1

2.5 ± 0.9

  1. Human PBMCs from healthy donors (n = 5) were cultured for 24 h in the absence or presence of 50 IU/mL rhIL-2, 10 ng/mL rhIL-15 or 35.7 ng/mL IL-15/SuIL-15Rα-IgG4 Fc complexes, and the expression of selected markers on CD8+ T cells was analyzed by flow cytometry. The percentages of CD8+ T cells that expressed the corresponding markers are listed in the table. dFc: IL-15/SuIL-15Rα-dFc; mFc: IL-15/SuIL-15Rα-mFc
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Microbial Cell Factories

ISSN: 1475-2859

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