Fig. 3

Characterisation of promoter activity with HBA module enzymes with a C-terminal GFP translational fusion. A Design of a degenerate σ⁷⁰ promoter library for characterisation with eGFP in microplate conditions, for full details see methods. B Promoter characterisation with HBA module enzyme fusions. Black bars represent OD₆₀₀, while coloured (pink/green/blue) bars represent eGFP fusions in terms of relative expression rate during exponential growth (GFP hr⁻¹). Data is representative of two technical readings and four biological repeats grown from single E. coli colonies. Error bars represent standard deviation