Fig. 8

Changes in transcript abundance of genes involved in MK-7 metabolic pathway. The biosynthesis pathway from glycerol to MK-7 in B. subtilis 168 was categorized into four modules I–IV. Enzymes: Module I (glycerol metabolism pathway, marked in red): GlpF glycerol uptake facilitator, GlpK glycerol kinase, GlpD glycerol-3-phosphate dehydrogenase, Tpi triosephosphate isomerase. Module II (MEP pathway, marked in blue): Dxs 1-deoxyxylulose-5-phosphate synthase, Dxr 1-deoxyxylulose-5-phosphate reductoisomerase, YacM 2-C-methylerythritol 4-phosphate cytidylyltransferase, IspE 4-diphosphocytidyl-2-C-methylerythritol kinase, YacN 2-C-methylerythritol 2,4-cyclodiphosphate synthase, YgfY 4-hydroxy-3-methylbut-2-enyl diphosphate synthase, YqfP 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, YpgA isopentenyl-diphosphate δ-isomerase, YqiD farnesyl diphosphate synthase. Module III (SA pathway, marked in purple): AroA 3-deoxy-7-phosphoheptulonate synthase, AroB 3-dehydroquinate synthase, AroC 3-dehydroquinate dehydratase, AroD shikimate dehydrogenase, AroK shikimate kinase, AroE 3-phosphoshikimate 1-carboxyvinyltransferase, AroF chorismate synthase. Module IV (MK-7 pathway, marked in green): MenF isochorismate synthase, MenD 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase, MenH 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase, MenC o-succinylbenzoate synthase, MenE o-succinylbenzoic acid-CoA ligase, MenB 1,4-dihydroxy-2-naphthoyl-CoA synthase, MenI 1,4-dihydroxy-2-naphthoyl-CoA hydrolase, MenA 1,4-dihydroxy-2-naphthoate heptaprenyltransferase, MenG demethylmenaquinone methyltransferase, HepS/HepT heptaprenyl diphosphate synthase component I/II. Other related pathways (branched metabolic pathway, marked in grey): Ldh lactate dehydrogenase, AlsS acetolactate synthase, AlsD acetolactate decarboxylase, AroH chorismate mutase, TrpE anthranilate synthase, PabB/PabA para-aminobenzoate synthase component I/II, DhbB bifunctional isochorismate lyase/aryl carrier protein. Abbreviations of metabolites: Gly glycerol, Gly-3P glycerol-3-phosphate, CDP-ME 4-(cytidine 5′-diphospho)-2-C-methylerythritol, CDP-MEP 2-phospho-4-(cytidine 5′-diphospho)-2-C-methylerythritol, PPA prephenate, PABA para-aminobenzoic acid, ADC 4-amino-4-deoxychorismate, DHDHB (2S,3S)-2,3-dihydro-2,3-dihydroxybenzoate, MG methylglyoxal, G1P glycerol-1-phosphate, G3P glyceraldehyde-3-phosphate, PEP phosphoenolpyruvate, PYR pyruvate, E4P erythrose 4-phosphate, DAHP 3-deoxy-arabino-heptulonate 7-phosphate, DHQ 3-dehydroquinate, DHS 3-dehydroshikimate, SA shikimate, S3P shikimate 3-phosphate, EPSP 5-O-(1-carboxyvinyl)-3-phosphoshikimate, CHA chorismate, DXP 1-deoxyxylulose-5-phosphate, MEP methyl-erythritol-4-diphosphate, HMBPP 1-hydroxy-2-methyl-2-butenyl 4-diphosphate, DMAPP dimethylallyl diphosphate, IPP isopentenyl diphosphate, GPP geranyl diphosphate, FPP farnesyl diphosphate, ICHA isochorismate, SEPHCHC 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate, SHCHC 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate, OSB 2-succinylbenzoate, OSB-CoA 2-succinyl benzoyl-CoA, DHNA-CoA 1,4-dihydroxy-2-naphthoyl-CoA, DHNA 1,4-dihydroxy-2-naphthoate, DMK 2-demethylmenaquinone, MK-7 menaquinone-7