Fig. 2
From: The cssR gene of Corynebacterium glutamicum plays a negative regulatory role in stress responses
CssR was negatively autoregulated. a, e β-Galactosidase analyses of the cssR promoter activity by using the transcriptional PcssR::lacZY chromosomal fusion reporter expressed in WT(pXMJ19), ΔcssR(pXMJ19), and ΔcssR(pXMJ19-cssR) strains in the presence of gentamicin (GEN) or CdCl2 (cadmium chloride) conditions. b, f Quantitative RT-PCR analyses of cssR expression in WT(pXMJ19), ΔcssR(pXMJ19), and ΔcssR(pXMJ19-cssR) strains under GEN or CdCl2 conditions. The mRNA levels were presented relative to the value obtained from WT(pXMJ19) cells without stress treatment. Relative transcript levels of WT(pXMJ19) strains without stress treatment were set at a value of 1.0. c, g The protein levels of NCgl1579 in WT and ΔcssR in the presence or absence of GEN and CdCl2. Lysates from stationary phase bacteria exposed to GEN or CdCl2 for 2 h were resolved by SDS-PAGE, and NCgl1579 was detected by immunoblotting using specific anti-NCgl1579 antibody. For the pellet fraction, RNA polymerase α (α-RNAP) was used as a loading control. Similar results were obtained in three independent experiments, and data shown were from one representative experiment done in triplicate. d, h Relative quantified data for protein levels by Image Lab. Quantified protein expression of Western blots in c and g. Densities of proteins were all justified with α-RNAP. Relative density ratios of C. glutamicum RES167 parental strains (WT) without stress were set at a value of 1.0. Data shown were the averages of three independent experiments, and error bars indicated the SDs from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001