In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain

Table 4 Comparison of titer and yield for reported S. cerevisiae muconic acid cell factories

Strain name	Genetic characteristics	Fermentation type (total h)	Carbon source	Titer (mg/L)	Yield (mg/g)	Refs
CEN.PK2-1C^aroED	ARO1^aroE^Δ, aroZ_Bth, asbF_Bth, aroY-C^iso	Batch (170 h)	D	1.56	0.78	[17]
MuA12	aro3Δ, zwf1Δ, ARO4^K229L, TKL1 aroZ_Pa, aroY_Ecl, HQD2_Ca	Batch (108 h)	D	141	3.53	[21]
MA4	ric1Δ, aro1Δ, ARO4^K229L, ARO1^D14091,D920A, TKL1, aroZ_Kp, aroY_Kp, HQD2_Ca	Batch (72 h)	D	2.7	33.57	[23]
MuA-5.01.1.02	TKL1, ARO1^aroE^Δ,aroZ_Pa, aroY_Ecl, HQD2_Ca	Fed-batch (240 h)	D	2.1	12.9	[24]
JTY4	zwf1Δ, TKL1, ARO3^K222L, ARO4^K229L,ARO1^aroE^Δ ARO1^K1370A, aroZ_Pa, TaGDC1, catA_Ar	Batch (120 h)	D	1244	31	[20]
Isolate 7	TKL1, aroZ_Pa, aroY-B, aroY-C^iso, aroY-D, catA_Ca	Fed-batch (120 h)	D	2008	13.4	[25]
Aro1_PEST-Hqd2(p6G)	aro3Δ, aro4Δ, ARO1-CLN2_PEST, PAD1, ARO4^K229L, aroB_Ec, aroD_Ec, aroZ_Pa, aroY_-C^iso, HQD2_Ca	Fed-batch (168 h)	D	5.1	58	[22]
ST8943	ARO4 ^K229L, ARO1^aroED, aroZ_Pa, aroY-C^iso, aroY-B, aroY-D, catA_Ca	Fed-batch (150 h)	D	20,800	66.2	[39]
Ic0.09-31	XK, PPP, ARO1^aroED, XylA_Bv aroZ_Nc, aroY_Ci,CatA_Cn	Batch (72 h)	DX	424	3.26	[14]
TN22	aro3Δ, ARO4^K229L, PAD1,TKL1, aroZ_Pa,, aroY-C^iso, HQD2_Ca	Batch (192 h)	^aD/X/DX	4030/4500/3840	33.6/37.3/30.7	This study

D glucose, X xylose
^aEthanol was added to the fermentation medium to address C2 auxotrophy of the pdc-strain. Fermentations were performed in rich YP medium, buffered with citrate buffer at pH 5.5 with an initial OD₆₀₀ 16

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