Fig. 6

Bacillus subtilis cell-free supernatant (B. subtilis CFS) increases the susceptibility of S. aureus to penicillin. a Survival percentage of implant-associated osteomyelitis mice infected by S. aureus pretreated with PBS (control) and B. subtilis CFS. All mice were treated with penicillin (80 mg/Kg/d) from the day challenged by S. aureus. N = 10/groups, *P < 0.05, Gehan-Breslow-Wilcoxon test. b Infection rate in surviving mice infected by S. aureus pretreated with PBS (control) and B. subtilis CFS on days 3 and 14 post-infection. N = 10/group, *P < 0.05, ns P > 0.05, Chi-square test. Quantification of S. aureus loading recovered from the implanted-tibia (c) and the needle (d) on days 3 and 14 post-infection. N = 10/group, **P < 0.01, ***P < 0.001, Mann-Whitney U test