Fig. 3

Characterization of surface-bound IsPETase^WT activity. a Activity and b whole-cell fluorescence of BL21(DE3) cells expressing IsPETase^WT within the LppOmpA surface display module compared to BL21(DE3) grown without expression vector. c Degradation of PET microparticles (15 g/L) measured via absorbance increase at 240 nm for wt IsPETase bound to the bacterial cell surface. Standard: 0.02 µM purified IsPETase^Austin