Table 1 Strains used in this study
Strains | Relevant characteristics | Source |
|---|---|---|
Escherichia coli | Â | Â |
 DH10B | Host strain for cloning | Invitrogen |
Saccharopolyspora erythraea | Erythromycin-producing strain | Lab stock |
Streptomyces hygroscopicus | Rapamycin-producing strain | Lab stock |
Schlegelella brevitalea | Â | Â |
 DSM 7029 | Wild type | Lab stock |
 104-1 | Epothilone producing strain | Lab stock |
 MMR2048 | Mutant strain of 104-1 with EPOAT4 replaced by EPOAT2 | This study |
 MMR2049 | Mutant strain of 104-1 with EPOAT4 replaced by EPOAT6 | This study |
 MMR2017 | Mutant strain of 104-1 with EPOAT4 replaced by EPOAT7 | This study |
 MMR2016 | Mutant strain of 104-1 with EPOAT4 replaced by EPOAT8 | This study |
 MMR2018 | Mutant strain of 104-1 with EPOAT4 replaced by RAPAT1 | This study |
 MMR2012 | Mutant strain of 104-1 with EPOAT4 replaced by ERYAT6 | This study |
 MMR2029 | Mutant strain of 104-1 with EPOAT4 replaced by EPOAT3 | This study |
 MMR2021 | Mutant strain of 104-1 with EPOAT4 replaced by EPOAT5 | This study |
 MMR2020 | Mutant strain of 104-1 with EPOAT4 replaced by EPOAT9 | This study |
 MMR2032 | Mutant strain of MMR2029 with EPOAT3 in EPOM4 replaced by aadA | This study |
 MMR2033 | Mutant strain of MMR2032 with aadA replaced by EPOAT3 (A185T) | This study |
 MMR2034 | Mutant strain of MMR2032 with aadA replaced by EPOAT3 (I209A) | This study |
 MMR2035 | Mutant strain of MMR2032 with aadA replaced by EPOAT3 (F310S) | This study |
 MMR2039 | Mutant strain of MMR2032 with aadA replaced by EPOAT3 (V383L) | This study |
 MMR2040 | Mutant strain of MMR2032 with aadA replaced by EPOAT3 (G426R) | This study |
 MMR2041 | Mutant strain of MMR2032 with aadA replaced by EPOAT3 (F310S-H308V) | This study |
 MMR2042 | Mutant strain of MMR2032 with aadA replaced by EPOAT3 (F310S-H308Y) | This study |
 MMR2055 | Mutant strain of 104-1 with EPOAT4 (S310F) | This study |
 MMR2037 | Mutant strain of 104-1 with EPODH9-ΨKR9-ER9 region deleted | This study |
 MMR2019 | Mutant strain of 104-1 with EPOER9 deleted | This study |
 MMR2038 | Mutant strain of MMR2019 with EPODH9 deleted | This study |
 MMR2053 | Mutant strain of MMR2038 with ΨKR9-KR9-linker deleted | This study |
 MMR2054 | Mutant strain of MMR2038 with ΨKR9-KR9-linker (EPOM9) replaced by ΨKR3-KR3-linker (EPOM3) | This study |
 MMR2044 | Mutant strain of 104-1 with EPOM4 replaced by EPOM7 | This study |
 MMR2024 | Mutant strain of 104-1 with EPOM4 replaced by ERYM6 | This study |
 MMR2027 | Mutant strain of 104-1 with EPOM4 replaced by RAPM4 | This study |
 MMR2026 | Mutant strain of 104-1 with EPOM4 replaced by RAPM10 | This study |