Fig. 6

Generation of productive epothilone PKSs through deletion of the inactive module 9 DH and ER domains and fusion of the ΨKR/KR domains. a Scheme for DH9-ΨKR9-ER9 deletion using pJM24. b PCR analysis with genomic DNA from 104-1, MMR2037, MMR2019 and MMR2038 using the primers JM51-F/R. c Production of epothilones. d Schemes for ER9 deletion using pJM25 and DH9 deletion using pJM26. The ~ 25-residue ΨKR9-KR9-linker is shown in red. e Diagram for ΨKR9-KR9-linker deletion and replacement. The ~ 25-residue ΨKR3-KR3-linker (EPOM3) is shown in green. Error bars represent one standard deviation from three biological replicates (n = 3)