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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Engineering the acyltransferase domain of epothilone polyketide synthase to alter the substrate specificity

Fig. 5

Site-directed mutagenesis of the AT domain. a Production and distributions of epothilones in parental and mutant strains. b Extracts from the parental and site-directed mutant strains were analysed by HPLC. 3 represents epothilone C, 4 represents epothilone D. c Identification of residues that are specifically related to AT domain substrates through protein sequence alignments. Statistical analysis was performed using Student’s t-test (***p < 0.001, ns not significant). Error bars represent one standard deviation from three biological replicates (n = 3)

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Microbial Cell Factories

ISSN: 1475-2859

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