Fig. 3

Replacing EPOAT4 with the AT domains specific to MCoA can increase the yield of epothilone C and eliminate epothilone D. a The module 4 AT domain of epothilone PKS (EPOAT4) was replaced with MCoA-specific AT domains (shown in red). b Epothilone production and epothilone C proportion from three engineered EPOAT4-swapped mutant strains (MMR2029, MMR2020, and MMR2021). c Extracts from the parental and mutant strains were analysed by HPLC. 3 represents epothilone C, 4 represents epothilone D. Statistical analysis was performed using Student’s t-test (***p < 0.001). Error bars represent one standard deviation from three biological replicates (n = 3)